Genome Sequences of the Oxytetracycline Production Strain Streptomyces rimosus R6-500 and Two Mutants with Chromosomal Rearrangements
نویسندگان
چکیده
The genome sequence of Streptomyces rimosus R6-500, an industrially improved strain which produces high titers of the important antibiotic oxytetracycline, is reported, as well as the genome sequences of two derivatives arising due to the genetic instability of the strain.
منابع مشابه
PRODUCTION OF OXYTETRACYCLINE BY ISOLATED WILD TYPE IRANIAN STREPTOMYCES RIMOSUS
Production of oxytetracycline by an isolated strain of Streptomyces rimosus from Iranian soil was investigated using a special fermentation medium. A comparative study was performed with standard strain PTCC 1144 using the following parameters optimum growth conditions with respect to time, pH, and different amounts of corn steep liquor. Surprisingly, the production yield of the wild strai...
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The 387kb linear plasmid pPZG101 of Streptomyces rimosus R6 can integrate into the chromosome or form a prime plasmid carrying the oxytetracycline biosynthesis cluster. The integration of plasmid pPZG101 into the linear chromosome of S. rimosus R6-501 in mutant MV25 was shown to be due to a single cross-over at a 4 bp common sequence. pPZG101 had integrated into a 250 kb DNA sequence that was r...
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This research is aimed to improve the oxytetracycline production from Streptomyces rimosus. Five relatively high oxytetracycline yielding strains were obtained by nitrosoguanidine mutation of the protoplast of initial oxytetracycline producing strain Streptomyces rimosus JH and then subjected to genome shuffling as parental strains. The protoplasts of the five parental strains were prepared and...
متن کاملThe temperate phages RP2 and RP3 of Streptomyces rimosus.
The oxytetracycline-producing Streptomyces rimosus strains R6-65 and R7 (ATCC 10970) are lysogenic for the two narrow-host-range phages RP2 and RP3. Both phages are released at low frequency from the lysogenic strains and form plaques on 'cured' S. rimosus strains. RP2 and RP3 are of similar shape with flexible tails and contain double-stranded DNA of about 70% G+C with cohesive ends (group B1 ...
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We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, a commercially important and clinically useful antibiotic.
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